비쑥의 휘발성물질에 의한 KB 세포의 세포사멸 유도
Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cell by TheEssential Oil of Artemisia Scoparia WALDST. et KITAMURA
차정단, 이생금, 이명섭, 차인호,
소속 상세정보
차정단 ( Cha Jeong-Dan ) - 연세대학교 치과대학 구강종양연구소
이생금 ( Li Sheng-Jin ) - 연세대학교 치과대학 구강종양연구소
이명섭 ( Lee Myeong-Seop ) - 연세대학교 치과대학 구강악안면외과학교실
차인호 ( Cha In-Ho ) - 연세대학교 치과대학 구강악안면외과학교실
KMID : 0829220090330020075
Abstract
Artemisia scoparia (A. scoparia), perennial herb is indigenous to Korea and has been traditionally used in liver damage. We investigated the effect of the essential oil obtained from A. scoparia on apoptosis of KB cells. Cytotoxicity and cellular DNA content were analyzed by MTT assay and flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. We found that the essential oil induced the apoptosis of the KB cells by concentrations of 0.4 to 0.2 mg/ml which was verified by DNA fragmentation, apoptotic bodies, and the sub-G0/G1 ratio. The essential oil also transient caspase-9 and caspase-3 activity and cleavage of PARP in KB cells for 24 h. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. In conclusion, we demonstrated that the essential oil of A. scoparia induces apoptosis in KB cells.
키워드
Artemisia scoparia;Essential oil;Apoptosis;Cell cycle;Caspase-3; PARP
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